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Protein-Protein Interactions: A Molecular Cloning Manual, Second Edition by Erica Golemis, Peter D. Adams

Protein-Protein Interactions: A Molecular Cloning Manual, Second Edition Erica Golemis, Peter D. Adams ebook
Publisher: Cold Spring Harbor Laboratory Press
Format: djvu
Page: 929
ISBN: 0879697237, 9780879697235

The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Molecular Cloning, a Laboratory manual. S: Molecular cloning: a laboratory manual Second edition. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. Why the "Second Edition" is the must-have guide for all pulmonologists and particle research scientists: STAY INFORMED - nine brand new chapters cover recent. Falciparum strain 3D7 genomic DNA and cloned into the expression plasmid pET-MCSIII [8] which adds a 6x His tag to the N-terminus of the protein (unpublished data). [9] Sambrook J, Fritsch EF, Maniatis T. Gambhir, “Imaging protein-protein interactions in living animals,” in Protein–Protein Interactions: A Molecular Cloning Manual, E. DNA: A model from DNA-binding studies of mutant proteins by surface plasmon resonance. The efficient packaging of vRNA also requires multipartite RNA-protein interaction of the leader RNA with the viral nucleocapsid (NC) domains of the Gag precursor (Pr55Gag), [7-9]. Protein-Protein Interactions: A Molecular Cloning Manual, Second Edition Erica Golemis, Peter D. Interestingly, while HIV-1 has been shown capable of packaging the genomic RNA of either human J Virol 1998, 72(4):2846-2854. At Publisher · View at Google Scholar · View at Scopus; R. Cold Spring Harbour: Cold Spring Harbour laboratory press 1989. Each protocol represents Practical manual of immunocytochemistry methods. By detecting the bioluminescence photons, we can characterize a variety of cellular and molecular processes, including protein interactions [20–23], protein degradation [24–26], and protease activity [27–29]. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL. An essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more.